Ability to apply principles of biology to derive energetics and stoichiometries in biological reactions.
Data analysis and interpretation in enzymatic and biological reactions.
Use and understanding of kinetic models in biochemical engineering.
Understanding the role of biochemical enginnering in technological fields such as pharmaceuticals and waste treatment.
Design of various types of bioreactors.
The students should refresh their knowledge in Microbiology.
Basics of microbiology, biochemistry and genetics.
Biochemical reaction stoichiometry, mass balances and energetics of half reactions.
Enzyme kinetics. The Michaelis-Menten and Briggs-Haldane models. Determination of kinetic parameters. Factors affecting enzymatic reactions (multiple substrates, co-enzymes, pH, temperature, reversible reactions). Enzyme inhibition (competitive, non-competitive, uncompetitive) and deactivation. Immobilized enzymes (mass transfer limitations, Thiele modulus, effectiveness factor).
Kinetics of microbial growth, substrate utilization and metabolic product generation.
The Monod model and comparison of various kinetic models. Factors affecting microbial growth. Sterilization and disinfection.
Bioreactor types (batch, fed-batch, CSTR). Bioreactor design and productivity optimization. Sequence of bioreactors. Biofilms (the ideal biofilm, biofilm models).
Bioseparations and down-stream processing (sedimentation, filtration, centrifugation, liquid-liquid extraction, chromatographic separations, electrophoresis, membranes, crystallization, drying).
LECTURES: 3 h/w
RECITATION: 2 h/w
Total Module Workload (ECTS Standards):
There is a final examination accounting for 100% of the mark.
1. Εισαγωγή στη Βιοχημική Μηχανική, Λυμπεράτου & Παύλου, Εκδόσεις Τζιόλα
2. Bioprocess Engineering, Shuler & Kargi, Prentice-Hall
3. Biochemical Engineering Fundamentals, Bailey & Ollis, 2nd edition, McGraw-Hill